PCR and Clone-Chip Ledgers

Many Wells Share One Schedule

Parallel wells multiply heat but not the programmed elapsed time.

Parallel wells multiply heat but not the programmed elapsed time. Exact arithmetic here means exact results for the stated model inputs; measured inputs still carry uncertainty and significant-figure limits.

highlighted = computed this step

Many wells share one schedule

The chip has 8 wells, but they run in parallel on one schedule.

elapsed time=120 s\text{elapsed time}=120\ \text{s}
Thermal cycle ledgerParallel wells share elapsed time while heat scales by well count.elapsed100 Jstep1200 Jwell9600 Jchipcycle ledger

Heat scales with well count

Total chip heat is 1200 joules per well times 8 wells, or 9600 joules.

1200⋅8=96001200\cdot8=9600
Thermal cycle ledgerParallel wells share elapsed time while heat scales by well count.elapsed100 Jstep1200 Jwell9600 Jchipcycle ledger