Coverage Acceptance Audit

Unique Read Bases Make Three-X Coverage

Coverage arithmetic starts from duplicate-filtered unique reads. Exact arithmetic here means exact results for the stated model inputs; measured inputs still carry uncertainty and significant-figure limits.

highlighted = computed this step

Unique reads create read bases

The duplicate-filter source gives 24 unique reads. Each read contributes 50 bases.

24⋅50=120024\cdot50=1200
Coverage acceptance gateUnique reads, read length, and genome size decide the exact coverage gate.unique=24readLength=50bases=1200genome=400coverage=3 xmin=3 xaccepted=1

The toy run reaches three-x coverage

Read bases 1200 over genome size 400 gives coverage 3x.

1200/400=31200/400=3
Coverage acceptance gateUnique reads, read length, and genome size decide the exact coverage gate.unique=24readLength=50bases=1200genome=400coverage=3 xmin=3 xaccepted=1