The acceptance gate accepts equality and rejects below-minimum coverage.

The acceptance gate accepts equality and rejects below-minimum coverage. Exact arithmetic here means exact results for the stated model inputs; measured inputs still carry uncertainty and significant-figure limits.

highlighted = computed this step

Equality at the minimum accepts

The minimum is 3x, and the accepted row computes exactly 3x.

3≥33\ge3
Coverage acceptance gateUnique reads, read length, and genome size decide the exact coverage gate.unique=24readLength=50bases=1200genome=400coverage=3 xmin=3 xaccepted=1

Two-x coverage is below the same minimum

The rejected contrast uses 16 unique reads, giving 2x and accepted bit 0.

16⋅50/400=2<316\cdot50/400=2<3
Coverage acceptance gateUnique reads, read length, and genome size decide the exact coverage gate.unique=16readLength=50bases=800genome=400coverage=2 xmin=3 xaccepted=0