A dilution standard is accepted only when it cites the matching threshold row. Exact arithmetic here means exact results for the stated model inputs; measured inputs still carry uncertainty and significant-figure limits.

highlighted = computed this step

A standard row cites its amplification table

The first standard cites the threshold row whose start is 64 and threshold cycle is 0.

Ct=0C_t=0
Serial dilution threshold ledgerEach twofold dilution shifts the threshold by one exact PCR cycle.starts=64,32,16,8Ct=0,1,2,3shift=0,1,2,3accepted=1

Equal-looking numbers are not enough

The source ref names the threshold-check slot and the field thresholdCycle.

source field=thresholdCycle\text{source field}=\text{thresholdCycle}
Serial dilution threshold ledgerEach twofold dilution shifts the threshold by one exact PCR cycle.starts=64,32,16,8Ct=0,1,2,3shift=0,1,2,3accepted=1