Cycle Time Budget - Biophysical Instrumentation III: Control, Detectors, and Throughput Step by Step

A PCR chip cycle is a checked sequence of thermal control windows. Exact arithmetic here means exact results for the stated model inputs; measured inputs still carry uncertainty and significant-figure limits.

highlighted = computed this step

A cycle is staged time

The staged control windows fill one cycle of 10 s.

t_{\text{cycle}}=10\ \text{s}

Cycle time repeats

For 6 cycles, the program time is 60 s. This is thermal scheduling, not reaction kinetics.

t_{\text{total}}=6t_{\text{cycle}}=60\ \text{s}