An ideal electrophoresis lane separates fragments because different mobilities reach different positions in the same run. Exact arithmetic here means exact results for the stated model inputs; measured inputs still carry uncertainty and significant-figure limits.
highlighted = computed this step
Lower mobility travels less far
The lower-mobility band has mobility index 1 and reaches 6 meters.
μlower=1,xlower=6m
Higher mobility travels farther in the same time
The higher-mobility band has mobility index 3 and reaches 18 meters.
μhigher=3,xhigher=18m
A middle mobility lands between them
At the same field and time, the middle mobility lands between the lower and higher bands.