mRNA is read in non-overlapping triplets; four bases in three positions give 64 codons.

highlighted = computed this step

Bases are read three at a time

The ribosome reads mRNA in non-overlapping groups of 3 bases. Each group is a codon, shown here bracketed over the strand it comes from.

codon=3 bases\text{codon} = 3\text{ bases}
CodonsThe mRNA is read in non-overlapping groups of three bases.mRNAAUGGCAUGCAAAUAAcodonsAUGGCAUGCAAAUAA

There are 64 possible codons

With 4 possible bases in each of 3 positions, there are 64 distinct codons.

43=644^3 = 64

The reading frame fixes where codons begin

Unless told otherwise we start at the first base — frame 0. Starting one or two bases later would regroup every codon and change the reading; that shift is the subject of a later lesson.

frame 0: AUGGCAUGCAAAUAA\text{frame } 0:\ \text{AUG}\,|\,\text{GCA}\,|\,\text{UGC}\,|\,\text{AAA}\,|\,\text{UAA}
Reading frameReading starts at the first base (frame 0).mRNAAUGGCAUGCAAAUAAcodonsAUGGCAUGCAAAUAA