A PCR threshold cycle is the first checked count that reaches the threshold. Exact arithmetic here means exact results for the stated model inputs; measured inputs still carry uncertainty and significant-figure limits.

highlighted = computed this step

The finite doubling table is the source

Start with 3 copies. The checked counts are 3,6,12,24,48.

3,6,12,24,483,6,12,24,48
PCR threshold crossingExact doubling pins the first cycle that reaches the threshold.c0=3c1=6c2=12c3=24c4=48start=3threshold=24Ct=3crossed=1accepted=1

Cycle three is the first crossing

The threshold is 24. Cycle 2 is below it, and cycle 3 reaches it.

12<24242412<24\quad24\ge24
PCR threshold crossingExact doubling pins the first cycle that reaches the threshold.c0=3c1=6c2=12c3=24c4=48start=3threshold=24Ct=3crossed=1accepted=1