Three coverage rows make the reject, equality, and pass boundary visible.
Three coverage rows make the reject, equality, and pass boundary visible. Exact arithmetic here means exact results for the stated model inputs; measured inputs still carry uncertainty and significant-figure limits.
highlighted = computed this step
Sixteen unique reads are below the minimum
The rejected row has 16 unique reads. With read length 50 and genome size 400, coverage is 2x.
16⋅50/400=2<3
Twenty-four unique reads meet the boundary
The boundary row has 24 unique reads and exactly 3x coverage, so equality accepts.
24⋅50/400=3≥3
Thirty-two unique reads clear the same gate
The high row has 32 unique reads, giving 4x coverage and accepted bit 1.