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Fluorescence Energy Readout

lower energy out

A simplified fluorescence readout compares excitation, emission, and count. Exact arithmetic here means exact results for the stated model inputs; measured inputs still carry uncertainty and significant-figure limits.

highlighted = computed this step

Emission is lower than excitation

The excitation energy is 12 J and the emitted training photon has 8 J, so the gap is 4 J.

Egap=4 JE_{\text{gap}}=4\ \text{J}
Fluorescence energyEmission carries less energy per training photon than excitation.exciteemittotal

Photon count scales total emitted energy

For 3 emitted training photons, the total emitted energy is 24 J. Joule-scale photon energies are schematic values.

Etotal=3Eemit=24 JE_{\text{total}}=3E_{\text{emit}}=24\ \text{J}
Fluorescence energyEmission carries less energy per training photon than excitation.exciteemittotal